Intern - Bioassay, Mol Bio & Protein Development, Summer 2020 - Tarryt

Compensation

: $87,420.00 - $133,750.00 /year *

Employment Type

: Full-Time

Industry

: Healthcare - Allied Health



Known for its scientific and operational excellence, Regeneron is a leading science-based biopharmaceutical company that discovers, invents, develops, manufactures, and commercializes medicines for the treatment of serious medical conditions. Regeneron commercializes medicines for eye diseases, high LDL-cholesterol, atopic dermatitis and a rare inflammatory condition and has product candidates in development in other areas of high unmet medical need, including rheumatoid arthritis, asthma, pain, cancer and infectious diseases.

These positions are in our Bioassay, Molecular Biology & Protein Development department within VI Antibody in Tarrytown, NY. There are 5 openings for Summer 2020. Each opening is described below.

Position 1:
This position is with the Therapeutic Proteins - Protein Development Group. The student will learn how to express protein in E. coli, isolate inclusion bodies, refold, & purify fluorophore-labeled complexes which are associated with human T-cells in the immune system. He or she will learn the chromatographic methods & equipment used to purify protein. Additionally, the intern will become familiar with some of the basic concepts & biochemistry of developing immunotherapies.

Position 2:
Antibody drug conjugates (ADCs) represent a rapidly growing therapeutic approach in the field of oncology. A thorough understanding of target and antibody intracellular trafficking remains a key driver in selection of both the target and the antibody in order to selectively deliver the cytotoxic payload. We developed a new method to simultaneously measure antibody binding, internalization, lysosomal localization, and the kinetics of antibody degradation in live cells utilizing a novel FRET-based cathepsisn B dependent cleavable fluorescent biosensor. The intern will apply this new method and develop new methods to quantitatively measure antibody recycling in living cells. First, the candidate will apply biosensor to measure antibody internalization and cleavage kinetics in lysosomes. Second, the intern will develop a fluorescence based method to quantitatively measure how much payload antibody can delivery for each cell. Finally, the intern will apply the combine method to a lot of targets in many tumor cell lines. Th intern will become familiar with genernal cell biology concepts, cell culture, cell imaging, high content screening, cell based assay development, and antibody interanlization and trafficking.

Position 3:
This internship will focus on the development of cell-based assays to measure the biological activity of antibodies and to support screening and lead candidate selection. This will involve mammalian cell culture, luciferase assays, flow cytometry, and confocal microscopy.

Position 4:
This position is in the Therapeutic Proteins department. The student will be part of a fast-paced bioassays group involved in cutting-edge work to develop novel therapeutics. This student will primarily focus on cell line engineering and cell-based assay development for ongoing therapeutics programs. He/she will also get the opportunity to learn about high-throughput screening using cell based assays. Good communication skills and passion for science are essential.

Position 5:
The intern will work in the department of Therapeutic Proteins, in the Bioassay, Molecular Biology & Protein Development group. The focus will be the production and characterization of amyloidogenic, fibrillizing protein reagents which are involved in neurodegenerative 'proteinopathies', which include proteins such as Tau, ?-synculcein, amyloid-?, TTR, & TDP-43 proteins. The intern will develop methods to form and isolate various aggregated states (monomer, oligomer, fibrils, etc.), which may be present in human samples or conditioned media. They will investigate parameters which affect the misfolding of fibrillizing proteins, and generate methods to isolate and study these proteins for research programs.

Start building your skills at Regeneron.

Must be pursuing a Bachelors, Masters, or PhD degree

Majoring in Biology, Molecular Biology, Biochemistry, Biosciences, Microbiology, or Bioengineering

1 year experience in science laboratory

Familiarity with methodology & basic concepts of protein expression & purification

Basic pippetting skills, mammalian cell culture technique, flow cytometry, cell-based bioassay development

Position 5- 1-2 years lab research experience. Buffer preparation, SDS-PAGE, Western blotting, absorbance spectroscopy and microscopy.Knowledge of protein expression in E.coli, and chromatography methods such as affinity, ion exchange, size exclusion, & reverse phase. Good Lab practices, attention to detail, including note taking and understanding of laboratory safety are critical.

General Intern Program Information:

Must be enrolled in, or accepted to, an academic program pursuing a Bachelor's, Master's, PhD or PharmD

Summer Program is full-time (~40 hrs/wk) for at least 10 weeks and is paid

Prefer demonstrated leadership in areas such as campus activities, clubs, sports or the community

Minimum GPA of 3.0

You will work with a specific manager on a project(s)

Enjoy weekly 1 hour Intern Program events: learn about other areas of the company from VPs and other employees, soft skills workshops, networking, volunteering, and more!

Present to your team on your summer project

Transportation and Housing:

Housing stipend available for those students who meet mileage requirements

A free shuttle is offered from the North White Plains or Tarrytown train stations to the Tarrytown, NY or Sleepy Hollow, NY campuses

Get to know other incoming Interns on our closed LinkedIn page when you accept an offer

This is an opportunity to join our select team that is already leading the way in the Pharmaceutical/Biotech industry. Apply today and learn more about Regeneron's unwavering commitment to combining good science & good business.

To all agencies: Please, no phone calls or emails to any employee of Regeneron about this opening. All resumes submitted by search firms/employment agencies to any employee at Regeneron via-email, the internet or in any form and/or method will be deemed the sole property of Regeneron, unless such search firms/employment agencies were engaged by Regeneron for this position and a valid agreement with Regeneron is in place. In the event a candidate who was submitted outside of the Regeneron agency engagement process is hired, no fee or payment of any kind will be paid.

Regeneron is an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, sexual orientation, gender identity, disability status, protected veteran status, or any other characteristic protected by law.
Associated topics: laboratory, mls, mlt, pathology, scientist, sterile processing technician, technician, technician iii, technician laboratory, technologist * The salary listed in the header is an estimate based on salary data for similar jobs in the same area. Salary or compensation data found in the job description is accurate.

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